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Moringa oleifera is one of the most famous plants in Afghanistan, Bangladesh, Bhutan, India, Maldives, Nepal, Pakistan, and Sri Lanka. It is known as miracle tree due to the fact of every parts of the plant such as roots, leaves, pods flowers, and seeds containing high nutritional value and medicinal benefits. Moringa oleifera seed’s oil extracted incorporates high antioxidants properties and come to be as a valuable sources of amino acids, protein, vitamins, beta carotene, and various phenolic compounds. Extraction of oil and determination of antioxidants in the oil could provide an excellent conceivable for commercialization particularly in pharmaceutical industries due to its pharmacological properties such as antiepileptic, antioxidant, antiflammatory, antihypertensive, antibacterial and antifungal. The purpose of this learn about have been to extract the Moringa oleifera seeds at different extraction time and ratio of seed to solvent and decided the amount of total flavonoid content (TFC) and total phenolic content (TPC) in the methanol extract. The extraction method was once carried out using Soxhlet extraction with methanol as a solvent for different ratio of seed to solvent (1:10, 1:5 and 3:10) and extraction time limit (2, 3, 4, 5 and 6 hours). The best percentages of total phenolic content have been 2027.07 (mg GAE/g of extract) at 3 hours of extraction time and seed to solvent ratio (1:10). However, the TFC values in Moringa oleifera seeds have been 99.72 (mg QE/g of extract weight) at 5 hours of extraction time limit and seed to solvent ratio (1:10). The greater values of TPC and TFC in methanol extract of M.
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